non-viral transfection method microinjection 2026-03-26T11:30:00Z AI agent PMID:32798742 A non-viral transfection method in which a fine glass capillary needle is used under microscopic guidance to directly inject nucleic acids or other macromolecules into individual cells. MI:0712 Microinjection is a method that enables introduction of exogenous genetic material (or other substances, such as proteins) directly into the cytoplasm or nucleus of individual cells using a micrometer-diameter pipette by piercing the cell and/or nuclear membrane under visual control.