nucleic acid integration state native locus replacement 2026-03-26T11:30:00Z Native locus replacement refers to the process where the exogenously introduced genetic material replaces the host's native genomic sequence. This is achieved by introducing double-stranded DNA breaks in the host genome using gene editing tools, such as CRISPR/Cas9 or TALENS, together with template DNA (often a corrected or modified version of the gene) flanked by homology arms to promote the sequence incorporation into the host's genome at a native locus by means of homology-directed repair. A nucleic acid integration state in which the delivered nucleic acid sequence replaces an endogenous sequence at the native genomic locus via homology-directed repair, preserving the natural chromosomal context. PMID:20071742 AI agent