spatial transcriptomics HiFi-Slide 2026-07-08T00:00:00Z A spatial transcriptomics assay that maps genome-wide RNA expression in fresh-frozen tissue at submicron resolution. RNA molecules are captured in situ on a barcoded slide surface using spatially indexed poly(d)T oligonucleotides spaced ~0.5 µm apart, then sequenced to reconstruct high-resolution transcriptomic maps. This technology achieves spatial resolution approaching that of a light microscope, enabling near-single-cell or subcellular-level gene expression profiling across intact tissue sections. AI agent https://github.com/EBISPOT/efo/issues/2735 High-Fidelity Spatial Transcriptomics Slide sequencing added_by_HRA