sends synaptic output to region receives synaptic input from neuron superior lateral protocerebrum superior medial protocerebrum calyx of adult mushroom body adult crepine dorsal anterior lateral neuron of the protocerebrum 2023-04-20T13:00:04Z https://github.com/FlyBase/drosophila-anatomy-developmental-ontology/issues/1622 FBbt:00059205 Pre-and postsynaptic sites were assessed by synaptotagmin or Dscam-GFP stainings, respectively. Synaptic input to the pioneer mushroom body alpha/beta neurons was visualized using GFP reconstitution across synaptic partners (GRASP). Function in the retrieval of long-term memory was assessed using an olfactory associative learning assay. Memory retrieval was impaired when neurotransmission was blocked using UAS-Ricin in the DAL neurons labelled with E0946-GAL4, G0338-GAL4 or G0431-GAL4. Note that DAL neurons are a subset of the neurons labeled by these GAL4 line lines, and so their identity was confirmed by immunostaining for DDC (FBgn0000422) (Chen et al., 2012). GRASP also used to show connectivity with MBON-alpha3 and MBON-beta'2mp (Wu et al., 2017). One of the neurons of the dorsal anterior lateral group of the protocerebrum (Xia et al., 2005). It projects its axons in three different regions: the superior dorsofrontal, the dorsolateral, and the inferior dorsofrontal protocerebrum domains. It establishes synaptic contacts with the pioneer mushroom body alpha/beta neurons in a small dorsal frontal domain of the mushroom body calyx (Chen et al., 2012). These neurons are involved in the retrieval of long-term memories (Chen et al., 2012) and are synapsed to mushroom body medial lobe arborizing neuron 4 and mushroom body vertical lobe arborizing neuron 3, which are also involved in long-term memory processing (Wu et al., 2017). VFB:FBbt_00059205 fly_anatomy.ontology DAL neuron mushroom body output neuron 3 mushroom body output neuron 14 neuron of the dorsal anterior lateral group of the protocerebrum