fasciculates with wedge commissure antennal mechanosensory and motor center B1 projection neuron antennal mechanosensory and motor center B1b projection neuron 2012-03-28T03:44:56Z AMMC-B1b neuron AMMC-B1b fly_anatomy.ontology Auditory function was determined measuring GCaMP activation in these cells in response to an acoustic stimuli. Subsets of AMMC-B1b neurons were labeled with the drivers: VT45599-GAL4, VT26006-GAL4, VT10277-GAL4 and VT30609-GAL4 (also labels AMMC-A2 neurons, distinguishable by its acoustic response). Single cell imaging with the first two drivers showed that the AMMC-B1b neurons are isomorphic. Presence of pre- and postsynaptic sites was determined by immunoreactivity to Dscam- and synaptotagmin-GFP localization, respectively. Connectivity between AMMC-B1b neurons and JO neurons in the AMMC was assessed using GFP reconstitution across synaptic membranes (GRASP). AMMC-B1a neurons were labeled using the VT45599-GAL4, VT26006-GAL4 or VT30609-GAL4 and a subset of JO neurons were labeled using L5219-LexA. Positive GRASP signals were also detected when using L5055-LexA to label AMMC-B1b and nan-GAL4 (FBti0101148) to label JO neurons. The expression pattern of L5055-LexA includes AMMC-B1b neurons as confirmed by its acoustic response to 100 Hz, 300 Hz or pulse song stimuli (Lai et al., 2012). nan-GAL4 labels almost all JO neurons (Kamikouchi et al., 2009). FBbt:00110122 VFB:FBbt_00110122 AMMC-B1b AMMC-B1b PN Projection neuron whose cell body is located dorsally to the antennal mechanosensory and motor center (AMMC) and innervates the AMMC zone B and both the ipsilateral and the contralateral inferior ventrolateral protocerebrum (IVLP) regions. These neurons receive input in the AMMC zone B from Johnston's organ neurons and establish presynaptic connections in the lateral region of the IVLP. These neurons are activated by an acoustic stimuli and are broadly tuned to respond to 100 Hz, 300 Hz or pulse song stimuli (Lai et al., 2012).