makes_use_of cryofixed tissue serial block face SEM (SBFSEM) cryogenic scanning electron microscopy cryogenic serial block face scanning electron microscopy 2026-03-13T13:54:56Z cryo-FIB-SEM serial block face imaging cryo-serial block face scanning electron microscopy serial block face cryo-SEM cryo FIB-SEM subtractive tomography cryo-serial block face SEM cryo FIB-SEM slice and view Cryogenic serial block-face scanning electron microscopy is a volumetric imaging technique in which the surface of a biological specimen preserved in its near-native, vitrified state is imaged by a scanning electron microscope, then ablated by a focused ion beam (FIB-SEM) to reveal a new surface, enabling three-dimensional ultrastructural reconstruction of cells and tissues without the artifacts introduced by chemical fixation or resin embedding.