substage of immediately preceded by developmental stage embryonic stage 14 dorsal closure stage embryonic stage 15 Stage 15 begins with the appearance of the second midgut constriction. During this stage the 1st and 3rd midgut constrictions form, dorsal closure is completed, and epidermal segmentation is accomplished. This stage ends when the intersegmental grooves can be distinguished at mid-dorsal level. Duration at 25 degrees C: approximately 100 minutes (680-780 minutes after egg laying). Temporal ordering number - 360. FBdv:00005332 FlyBase_development_CV