substage of immediately preceded by immediately precedes larval stage third instar larval stage prepupal stage Temporal ordering number - 500. The prepupal stage begins at puparium formation and ends when larval/pupal apolysis is complete, as indicated by the completion of imaginal head sac eversion and the expulsion of the oral armature of the larva. Duration at 25 degrees C: approximately 12 hours. (120-132.2 hours after egg laying; 0-12.2 hours after puparium formation). FBdv:00005342 FlyBase_development_CV pupal stage P-stage third instar larval stage L2 late third instar larval stage life stage