substage of immediately preceded by developmental stage larval stage third instar larval stage prepupal stage prepupal stage P1 Extends from puparium formation through tanning of the pupal cuticle. Posterior spiracles and ridge between anterior spiracles tan orange. Wriggling stops completely. Puparium becomes brown to the unaided eye. Duration at 25 degrees C: approximately 20 minutes. (120-120.3 hours after egg laying; 0-0.3 hours after puparium formation). pupal stage P1 white prepupa FBdv:00005343 Temporal ordering number - 510. puparium formation white puparium stage FlyBase_development_CV pupal stage P0 third instar larval stage L2 late third instar larval stage