substage of immediately preceded by developmental stage prepupal stage P4 prepupal stage P4(ii) pupal stage P5 pupal stage P5(i) FlyBase_development_CV Legs and wings reach full extension along abdomen. Enlarged initial segments of anterior pair Malpighian tubules move from thorax into abdomen. Translucent patch that lacks adhering fat body cells becomes discernible in the middle of the eye region. Pair of white Malpighian tubules becomes visible dorsally in the abdomen. Duration at 25 degrees C: approximately 66 minutes. (132.2-133.3 hours after egg laying; 12.2-13.3 hours after puparium formation). FBdv:00005351 Temporal ordering number - 590. Malpighian tubules migrating