immediately preceded by immediately precedes larval stage third instar larval stage adult stage adult stage A1 P-stage FlyBase_development_CV Temporal ordering number - 490. DISAMBIGUATION: In Drosophila lab vernacular, the term 'pupal stage' is often used to refer to the entire period from formation of the puparium to eclosion. However, this does not correspond to the standard usage of 'pupal stage' for cyclorrhaphous flies (for discussion see: FBrf0087128). Briefly: formation of the puparium (hardening of the larval cuticle) marks the beginning of the pre-pupal stage. The pupal stage begins following pupal/larval apolysis - detachment of the larval epidermis from the puparium. In Drosophila, the outward sign of the completion of apolysis is the eversion of the head an expulsion of the larval mouthparts (FBrf0036849). We use P-stage to refer to the stage from pupariation to eclosion, and restrict pupal stage to its standard usage. FBdv:00007001 The stage of the Drosophila life-cycle from the formation of the puparium (beginning of the prepupal stage) to eclosion. pupal stage metamorphosis 2008-06-05T04:51:40Z third instar larval stage L2 late third instar larval stage life stage immature adult stage