substage of immediately preceded by immediately precedes larval stage second instar larval stage third instar larval stage The third larval instar begins at the second larval molt and ends at puparium formation. Anterior spiracles are open to the outside; posterior spiracles have three openings each, and four groups of large branched hairs. The salivary glands extend to the second abdominal segment, with cells larger in the posterior than in the anterior of the gland. Mouth hooks typically have 9-12 teeth. About 24 hours before pupariation (at 25 degree C), larvae stop feeding and climb away from their food. Duration at 25 degrees C: approximately 48 hours (72-120 hours after egg laying; 48-96 hours after hatching). FBdv:00005339 L3 third instar larva FlyBase_development_CV UBERON:8000002 Temporal ordering number - 430. prepupal stage P-stage life stage