substage of immediately preceded by developmental stage wandering third instar larval stage third instar - partially cleared gut stage third instar - cleared gut stage FlyBase_development_CV Temporal ordering number - 467. This is commonly assayed by adding 0.5% bromophenol blue to the food and looking for wandering third instar larvae with guts lacking any blue stain (Andres and Thummel, 1994). Larvae at this stage are mainly in chromosomal puff stages 7-9 and pupate within 1-6 hours (Andres and Thummel, 1994. FBdv:00007021 The substage of the wandering third instar larval stage when the larva has cleared its gut contents.