genomic assay whole genome sequencing assay WGS WGS genomics PMID:23095910 A DNA sequencing assay that intends to provide information about the sequence of an entire genome of an organism. PMID:25827230 WGS permits comprehensive sequencing of introns and exons, whereas whole exome sequencing (WES) allows deeper sequencing of exonic regions at a lower cost. Due to the large number of genetic variants found in each genome, it is necessary to use filtering approaches to distinguish deleterious from benign variants. WES has been used successfully to identify novel genetic causes of primary immunodeficiency. Complex structural variations and non-Mendelian disorders remain challenges for WGS.