454 Genome Sequencer 454 Genome Sequencer FLX+ 2023-11-28T18:23:15.862Z https://www.thelabworldgroup.com/product/roche-gs-454-flx-dna-sequencer/ 454 GS FLX+ to be replace with external ontology term A 454 Genome Sequencer that performs pyrosequencing and comprises both optics and fluidics subsystems and can be used for longer reads when used with the long-read Sequencing Kit XL+. Planned Obsolescence: this term is a placeholder for a term requested in another ontology. Once the appropriate ontology term is available, this term’s identifier will be obsoleted with a “term replaced by” id of the other term. The fluidics subsystem ensures accurate reagent dispensing. It consists of a reagents cassette (which holds the reagent containers), a sipper manifold, pumps, valves, and debubblers. The fluidics subsystem flows the sequencing reagents across the wells of the PicoTiterPlate device, and moves the spent reagents from the PicoTiterPlate device to the waste receptacle. The optics subsystem consists of a CCD camera and a camera controller. The camera captures the light emitted in the wells of the PicoTiterPlate device during each step of the sequencing cycle, and sends the digital images to the computer subsystem for processing. The computer controls the other Sequencer subsystems, and processes the digital images sent by the camera to extract the DNA sequence information. https://en.wikipedia.org/wiki/454_Life_Sciences