is_specified_output_of derives from derives from primary cultured cell cultured cell secondary cultured cell The term 'secondary cell culture' is generally used in biological texts/protocols to refer to any culture following an initial passage. We include it here because there are often a number of passages between a primary culture and the establishment of a stable, homogenous cell line. Such cultures are considered to be 'secondary cultures' but not 'cell lines' during this intermediate passaging/selection period between their derivation from a 'primary cell culture' and derivation into a 'cell line', which is a more specific type of secondary culture. PERSON: Matthew Brush A secondary cultured cell has been passaged in culture or is a descendant of such a cell that is derived through propagation in culture. Person: Matthew Brush A cultured cell that has been passaged or derives from a cell that has been passaged in culture. secondary cultured cell cell culture splitting metadata complete