part of mitotic sister chromatid segregation mitotic cell cycle mitotic metaphase chromosome alignment https://github.com/geneontology/go-ontology/issues/25547 biological_process A chromosome localization process whereby chromosomes are positioned in a specific order and orientation at the metaphase plate (spindle equator), during mitotic chromosome segregation. This alignment ensures that each daughter cell will receive the correct number of chromosomes during cell division. GO:0007080 metaphase chromosome alignment mitotic cell cycle process