negatively regulates DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity enzyme inhibitor activity DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) inhibitor activity GO:0008657 molecular_function Binds to and stops, prevents or reduces the activity of ATP-hydrolyzing DNA topoisomerase. ATP-hydrolyzing DNA topoisomerase catalyzes the DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined; product release is coupled to ATP binding and hydrolysis; changes the linking number in multiples of 2. DNA gyrase inhibitor activity ATPase inhibitor activity DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) regulator activity