type II site-specific deoxyribonuclease activity https://github.com/geneontology/go-ontology/issues/30193 type II restriction enzyme activity molecular_function Catalysis of the endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates and 3' hydroxyls. Cleavage is dependent on the presence in the DNA of a specific recognition site; cleavage occurs at or very near this recognition site. GO:0009036 MetaCyc:3.1.21.4-RXN EC:3.1.21.4 restriction endodeoxyribonuclease activity DNA endonuclease activity, producing 5'-phosphomonoesters