thiamine diphosphate biosynthetic process thiamine pyrophosphate biosynthetic process thiamine diphosphate biosynthesis thiamin pyrophosphate biosynthesis thiamin diphosphate biosynthetic process thiamine diphosphate anabolism thiamine diphosphate formation thiamin pyrophosphate biosynthetic process thiamine diphosphate synthesis TPP biosynthesis thiamine pyrophosphate biosynthesis TPP biosynthetic process GO:0009229 biological_process The chemical reactions and pathways resulting in the formation of thiamine diphosphate, a derivative of thiamine (vitamin B1) which acts as a coenzyme in a range of processes including the Krebs cycle. thiamine diphosphate metabolic process thiamine-containing compound biosynthetic process organophosphate biosynthetic process