negatively regulates regulation of triglyceride biosynthetic process negative regulation of triglyceride biosynthetic process biological_process negative regulation of triacylglycerol biosynthetic process Any process that decreases the rate, frequency, or extent of triglyceride biosynthesis. Triglyceride biosynthesis is the collection of chemical reactions and pathways resulting in the formation of triglyceride, any triester of glycerol. GO:0010868 triglyceride biosynthetic process negative regulation of lipid biosynthetic process biological regulation negative regulation of triglyceride metabolic process