tRNA (cytidine) methyltransferase activity tRNA (cytidine-N5)-methyltransferase activity https://github.com/geneontology/go-ontology/issues/24771 https://github.com/geneontology/go-ontology/issues/26109 https://github.com/geneontology/go-ontology/issues/30193 Reactome:R-HSA-6782388 RHEA:42940 transfer ribonucleate cytosine 5-methyltransferase activity RHEA:51164 RHEA:42944 RHEA:51160 tRNA (cytidine-5-)-methyltransferase activity RHEA:42948 RHEA:61488 S-adenosyl-L-methionine:tRNA (cytosine-5-)-methyltransferase activity Reactome:R-HSA-8932765 RHEA:51176 RHEA:51172 RHEA:53076 RHEA:61468 RHEA:21124 Reactome:R-HSA-6785438 transfer RNA cytosine 5-methyltransferase activity RHEA:54136 RHEA:61988 RHEA:61584 GO:0016428 RHEA:51180 molecular_function RHEA:42952 Reactome:R-HSA-6785409 Reactome:R-HSA-6782419 tRNA (cytosine-5-)-methyltransferase activity Catalysis of the reaction: a cytidine in tRNA + S-adenosyl-L-methionine = a 5-methylcytidine in tRNA + S-adenosyl-L-homocysteine + H+. This modification can occur on several residues, including cytidine(34), cytidine(40), cytidine(48), and cytidine(49). RHEA:54140 EC:2.1.1.202 RHEA:42956