S-adenosylmethionine-dependent methyltransferase activity tRNA (uridine) methyltransferase activity tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent https://github.com/geneontology/go-ontology/issues/26118 https://github.com/geneontology/go-ontology/issues/30193 molecular_function tRNA:m(5)U54-methyltransferase activity ribothymidyl synthase activity GO:0030697 S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity tRNA (uracil(54)-C(5))-methyltransferase activity, S-adenosyl methionine-dependent tRNA (uracil-5-)-methyltransferase activity MetaCyc:TRNA-URACIL-5--METHYLTRANSFERASE-RXN transfer RNA uracil methylase activity M5U-methyltransferase activity transfer RNA uracil 5-methyltransferase activity EC:2.1.1.35 tRNA uracil 5-methyltransferase activity tRNA:m5U54-methyltransferase activity S-adenosyl methionine-dependent tRNA (m5U54) methyltransferase activity Catalysis of the reaction: S-adenosyl-L-methionine + uridine54 in tRNA = 5-methyluridine54 in tRNA + H+ + S-adenosyl-L-homocysteine. RUMT RUMT activity S-adenosyl-L-methionine:tRNA (uracil-5-)-methyltransferase activity GO:0009021 RHEA:42712