steroid 7-alpha-hydroxylase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen 25-hydroxycholesterol 7-alpha-hydroxylase activity https://github.com/geneontology/go-ontology/issues/28070 https://github.com/geneontology/go-ontology/issues/29041 EC:1.14.14.29 cholest-5-ene-3beta,25-diol,NADPH:oxygen oxidoreductase (7alpha-hydroxylating) activity CYP7B1 oxysterol 7alpha-hydroxylase activity molecular_function MetaCyc:RXN-7981 GO:0033783 MetaCyc:RXN-7980 RHEA:19041 Catalysis of the reactions: 25-hydroxycholesterol + O2 + reduced [NADPH--hemoprotein reductase] = 7alpha,25-dihydroxycholesterol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]. Also converts (25R)-cholest-5-ene-3beta,26-diol to (25R)-cholest-5-en-3beta,7alpha,26-triol. 25-hydroxycholesterol 7alpha-monooxygenase activity RHEA:24308 CYP7B1