DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity DNA negative supercoiling activity https://github.com/geneontology/go-ontology/issues/15575 https://github.com/geneontology/go-ontology/issues/28520 Note that this term was reinstated from obsolete. molecular_function DNA gyrase activity Catalytic introduction of negative supercoils into a DNA molecule or region thereof. In bacteria, negative supercoils are only introduced by DNA gyrase, a type II topoisomerase, but not all DNA gyrases are capable of introducing supercoils. In bacteria, the level of supercoiling varies widely between species and has been characterized properly in only a handful of organisms. The best characterized enzyme, from E.coli, is exceptionally proficient at supercoiling and this ability is not representative of all bacteria. DNA-gyrase activity GO:0034335