part of methylthiotransferase activity tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity https://github.com/geneontology/go-ontology/issues/29839 https://github.com/geneontology/go-ontology/issues/30193 RHEA:37075 GO:0035598 molecular_function MetaCyc:RXN-14531 EC:2.8.4.5 t6A methylthiotransferase activity Catalysis of the reaction: N(6)-L-threonylcarbamoyladenosine(37) in tRNA + [sulfur carrier]-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + [sulfur carrier]-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H+. N6-threonylcarbomyladenosine methylthiotransferase activity bf Reactome:R-HSA-6786571 2010-11-12T01:28:32Z tRNA methylthiolation catalytic activity, acting on a tRNA