TIM22 mitochondrial import inner membrane insertion complex GO:0042721 cellular_component A multi-subunit complex embedded in the mitochondrial inner membrane that mediates the inner membrane insertion of multi-transmembrane spanning proteins that contain internal targeting elements. In yeast cells, TIM22 is a 300-kDa complex, consisting of four membrane integral subunits, Tim22, Tim54, Tim18 and Sdh3, and a peripheral chaperone complex consisting of the small TIM proteins, Tim9-Tim10-Tim12. mitochondrial protein translocase complex Tim22 complex mitochondrial inner membrane protein insertion complex inner mitochondrial membrane protein complex