part of tRNA-specific ribonuclease activity RNA endonuclease activity producing 5'-phosphomonoesters, hydrolytic mechanism tRNA 3'-end processing 3'-tRNA processing endoribonuclease activity https://github.com/geneontology/go-ontology/issues/21383 Catalysis of the endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule. EC:3.1.26.11 RNase Z activity GO:0042781 tRNA 3' endonuclease activity 3 tRNase activity tRNA 3 endonuclease activity 3' tRNase activity ribonuclease Z activity tRNAse Z 3' tRNA processing endoribonuclease activity MetaCyc:3.1.26.11-RXN molecular_function