aminoacyl-tRNA ligase activity pyrrolysyl-tRNA synthetase activity https://github.com/geneontology/go-ontology/issues/30193 MetaCyc:6.1.1.26-RXN Catalysis of the reaction: ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl). PylRS RHEA:19277 EC:6.1.1.26 GO:0043767 pyrrolysine-tRNA ligase activity molecular_function