hydrolase activity, hydrolyzing O-glycosyl compounds [protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/L-threonine O-N-acetyl-alpha-D-glucosaminase activity https://github.com/geneontology/go-ontology/issues/27560 https://github.com/geneontology/go-ontology/issues/30193 molecular_function RHEA:48876 GO:0102571 RHEA:48892 EC:3.2.1.169 MetaCyc:RXN-15215 Catalysis of the reaction: 3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl/L-threonyl-[protein] + H2O = L-seryl//L-threonyl-[protein] + N-acetyl-D-glucosamine. catalytic activity, acting on a protein