Thiamin is pyrophosphorylated Cytosolic thiamin pyrophosphokinase (TPK) catalyzes the reaction of thiamin and ATP to form thiamin pyrophosphate (TPP) (thiamin diphosphate) and ADP. TPP is an active cofactor for transketolase, pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase, enzymes involved in glycolysis and energy production. The gene encoding the human enzyme has been cloned and its protein product has been shown to have TPK activity (Nosaka et al. 2001; Zhao et al. 2001). Its homodomeric structure and association with Mg++ are inferred from properties of the homologous yeast enzyme (Baker et al. 2001). EC Number: 2.7.6.2 Pubmed11342111 Pubmed11342117 Pubmed11435118 Reactome Database ID Release 43196761 Reactome, http://www.reactome.org ReactomeREACT_11233