GAR + 10-Formyl-THF => FGAR + THF 5-phosphoribosylglycinamide (GAR) + 10-formyl-tetrahydrofolate => 5'-phosphoribosylformylglycinamide (FGAR) + tetrahydrofolate Authored: D'Eustachio, P, 2004-02-12 16:37:00 EC Number: 2.1.2.2 Edited: D'Eustachio, P, Jassal, B, 0000-00-00 00:00:00 Pubmed11381136 Pubmed12450384 Pubmed18388293 Pubmed2147474 Reactome Database ID Release 4373813 Reactome, http://www.reactome.org ReactomeREACT_1509 The irreversible transfer of a formyl group to cytosolic 5-phosphoribosylglycinamide (GAR) to form 5'-phosphoribosylformylglycinamide (FGAR) is catalyzed by the phosphoribosylglycinamide formyltransferase domain of the trifunctional protein, "phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase" (GART) (Aimi et al. 1990; Zhang et al. 2002). Fluoresence microscopy studies of cultured human cells have shown that GART is cytosolic and suggest that it may co-localize with other enzymes of de novo IMP biosynthesis under some metabolic conditions (Gooljarsingh et al. 2001; An et al. 2008).