TMP, (d)UMP, uridine 2' monophosphate, uridine 3' monophosphate thymidine, uridine, deoxyuridine TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O => thymidine, deoxyuridine, or uridine + orthophosphate [NT5M] 5'3'-Nucleotidase, mitochondrial (NT5M) is the major nucleotidase of human mitochondria, catalyzing the hydrolysis of TMP, uridine 2'-, 3'-, and 5'-monophosphates, and dUMP to yield the corresponding (deoxy)nucleosides and orthophosphate. It may play a central role in "substrate cycles" to regulate mitochondrial deoxynucleotide levels, especially in non-dividing cells (Rampazzo et al. 2000; Gallinaro et al. 2002). The active form of the enzyme is a homodimer, with an absolute requirement for Mg++ (Rampazzo et al. 2000; Rinaldo-Matthis et al. 2002). EC Number: 3.1.3.5 Pubmed10899995 Pubmed12124385 Pubmed12352955 Reactome Database ID Release 43109514 Reactome, http://www.reactome.org ReactomeREACT_1389