(d)CMP (deoxy)cytidine (2'-deoxy)cytidine + ATP => (d)CMP + ADP [DCK] Cytosolic deoxycytidine kinase (DCK) catalyzes the reactions of cytidine or deoxycytidine with ATP to form CMP or dCMP and ADP. The enzyme is a homodimer (Sabini et al. 2003). Although a chimeric deoxycytidine kinase - green fluorescent protein expressed at high levels in cultured cells localized to nuclei, endogenous protein is primarily cytosolic (Hatzis et al. 1998). Despite its name, the enzyme has a broad substrate specificity, acting on cytidine, deoxycytidine, deoxyguanosine, and deoxyadenosine (Bohman and Eriksson 1988; Datta et al. 1989a, b; Sarup et al. 1989; Usova and Eriksson 2002). While ATP functions efficiently as a phosphate donor, other nucleoside triphosphates, notably UTP, function efficiently as phosphate donors in vitro and may function in this way in vivo as well (Shewach et al. 1992). Pubmed12429345 Pubmed12808445 Pubmed1406603 Pubmed1996353 Pubmed2539852 Pubmed2542307 Pubmed2548513 Pubmed2844225 Pubmed9804782 Reactome Database ID Release 4373598 Reactome, http://www.reactome.org ReactomeREACT_1547 phosphorylation of 2'-Deoxycytidine to 2'-Deoxycytidine 5'-phosphate