bicarbonate and glutamine combine to form carbamoyl phosphate Authored: Jassal, B, 2003-06-17 09:00:25 EC Number: 6.3.5.5 Edited: D'Eustachio, P, 0000-00-00 00:00:00 L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate Pubmed4684686 Pubmed8619816 Reactome Database ID Release 4373577 Reactome, http://www.reactome.org ReactomeREACT_478 The synthesis of carbamoyl phosphate from glutamine, bicarbonate, and ATP is catalyzed by the carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity of cytosolic trifunctional CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) protein (Ito and Uchino 1973; Iwahana et al. 1996). The purified human protein is active in several different oligomerization states, as is its Syrian hamster homologue. The most abundant form of the latter is a hexamer, and the active human protein is annotated as a hexamer by inference (Ito and Uchino 1973; Lee et al. 1985). has a Stoichiometric coefficient of 2