(deoxy)guanosine, (deoxy)inosine (d)GMP, (d)IMP (d)GMP or (d)IMP + H2O => (2'-deoxy)guanosine or (2'-deoxy)inosine + orthophosphate [NT5C2] Cytosolic purine 5'-nucleotidase (NT5C2) catalyzes the hydrolysis of GMP, dGMP, IMP, and dIMP to yield the corresponding nucleosides and orthophosphate (Bianchi and Spychala 2003; Spychala et al. 1988). The active form of the enzyme is a tetramer and has an absolute requirement for magnesium ions (Spychala et al. 1988; Wallden et al. 2007). Consistent with the biochemicalproperties of purified enzyme, cultured cells overexpressing the enzyme from a recombinant DNA clone showed enhanced activity against inosine and guanosine monophosphates, but not adenosine monophosphate (Gazziola et al. 2001; Sala-Newby et al. 2000). EC Number: 3.1.3.5 Pubmed10766785 Pubmed11083867 Pubmed12947102 Pubmed17405878 Pubmed2848805 Reactome Database ID Release 4374248 Reactome, http://www.reactome.org ReactomeREACT_1336