CERT [ER] + ceramide [ER] => CERT:ceramide [ER] Authored: D'Eustachio, P, 2009-08-20 Edited: D'Eustachio, P, 2009-08-20 Pubmed14685229 Pubmed16895911 Pubmed18165232 Pubmed18184806 Reactome Database ID Release 43429699 Reactome, http://www.reactome.org ReactomeREACT_19228 Reviewed: Hannun, YA, Luberto, C, 2009-11-18 Reviewed: Jassal, B, 2009-08-20 “CERT” (ceramide transfer protein), an isoform of COL4A3BP, mediates the translocation of ceramides from the endoplasmic reticulum (ER) membrane to the membrane of the Golgi apparatus. Immunoprecipitation experiments suggest that “CERT” is associated with the ER membrane as part of a complex with PPM1L (protein phosphatase 1-like) (Saito et al. 2008) and VAPA or VAPB (VAMP-associated proteins A or B) (Kawano et al. 2006). The carboxyterminal “START” domain of “CERT” protein specifically binds ceramides (Hanada et al. 2003; Kudo et al. 2008). “CERT” protein binds ceramide associated with the endoplasmic reticulum membrane ceramide:CERT:PPM1L:VAPA/B trimer CERT:PPM1L:VAPA/B trimer