CERT:ceramide [ER] => ceramide [Golgi] + CERT [ER] Authored: D'Eustachio, P, 2009-08-20 Edited: D'Eustachio, P, 2009-08-20 Pubmed14685229 Pubmed16571669 Pubmed16895911 Pubmed18165232 Reactome Database ID Release 43429683 Reactome, http://www.reactome.org ReactomeREACT_19225 Reviewed: Hannun, YA, Luberto, C, 2009-11-18 Reviewed: Jassal, B, 2009-08-20 “CERT” (ceramide transfer protein), associated with the cytosolic face of the endoplasmic reticulum (ER) in a complex with VAPA or VAPB (VAMP-associated proteins A or B) (Kawano et al. 2006) and PPM1L (protein phosphatase 1-like) (Saito et al. 2008), can bridge the gap between the ER and the Golgi apparatus via its PH domain and transfer a molecule of ceramide extracted from the ER membrane to the Golgi (Hanada et al. 2003; Saito et al. 2008). “CERT”-mediated ceramide transfer is positively regulated by OSBP (oxysterol binding protein), by an unknown mechanism (Perry and Ridgway 2006). “CERT” protein releases its bound ceramide into the membrane of the Golgi apparatus ceramide:CERT:PPM1L:VAPA/B trimer CERT:PPM1L:VAPA/B trimer