Acetyl-CoA + H2O + Oxaloacetate => Citrate + CoA EC Number: 2.3.3.1 Mitochondrial citrate synthase dimer catalyzes the irreversible reaction of acetyl-CoA, water, and oxaloacetate to form citrate and coenzyme A. This reaction is the entry point of two-carbon units into the citric acid cycle. The reaction is subject to allosteric regulation. The gene encoding the human enzyme has been cloned (Goldenthal et al. 1998), but the enzyme has not been characterized in detail - its properties are inferred from those of the well-studied homologous pig enzyme (e.g., Morgunov and Srere 1998). Pubmed9792662 Pubmed9809442 Reactome Database ID Release 4370975 Reactome, http://www.reactome.org ReactomeREACT_1282