Synthesis of o-acetylcholine Authored: Mahajan, SS, 2008-01-14 16:01:52 Cho is acetylated to AcCho by CHAT EC Number: 2.3.1.6 In the cytosol, choline O-acetyltransferase (CHAT) acetylates choline (Cho) to produce acetylcholine (AcCho) (Toussaint 1992).<br><br>AcCho is synthesised in the cytoplasm of cholinergic neurons from acetyl-CoA and Cho by CHAT enzyme. Pubmed1339386 Reactome Database ID Release 43264622 Reactome, http://www.reactome.org ReactomeREACT_15484