PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the late endosome membrane At the late endosome membrane, the PAS complex, consisting of FYVE finger-containing phosphoinositide kinase (PIKFYVE), yeast VAC14 homologue (VAC14), and polyphosphoinositide phosphatase aka SAC3 (FIG4), binds to the membrane via PIKFYVE's FYVE finger (Sbrissa et al. 2002, Cao et al. 2007). The PIKFYVE kinase component phosphorylates phosphatidylinositol 3-phosphate (PI3P) to phosphatidylinositol 3,5-bisphosphate PI(3,5)P2 (Sbrissa et al. 1999). The PAS complex is present in the cytosol and is recruited to the membrane (Sbrissa et al. 2007). Authored: Williams, MG, 2011-10-18 EC Number: 2.7.1.150 Edited: Williams, MG, 2011-08-12 Pubmed10419465 Pubmed11706043 Pubmed17556371 Pubmed17651088 Reactome Database ID Release 431675910 Reactome, http://www.reactome.org ReactomeREACT_120833 Reviewed: Wakelam, Michael, 2012-05-14