PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the Golgi membrane At the Golgi membrane, the PAS complex, consisting of FYVE finger-containing phosphoinositide kinase (PIKFYVE), yeast VAC14 homologue (VAC14), and polyphosphoinositide phosphatase aka SAC3 (FIG4), binds to the membrane via PIKFYVE's FYVE finger (Sbrissa et al. 2002). The PIKFYVE kinase component phosphorylates phosphatidylinositol 3-phosphate (PI3P) to phosphatidylinositol 3,5-bisphosphate PI(3,5)P2 (Sbrissa et al. 1999, McEwen et al. 1999). The PAS complex is present in the cytosol and is recruited to the membrane (Sbrissa et al. 2007). VAC14 acts as a scaffolding protein via its C-terminal domain (Sbrissa et al. 2008). Authored: Williams, MG, 2011-10-18 EC Number: 2.7.1.150 Edited: Williams, MG, 2011-08-12 Pubmed10419465 Pubmed10567352 Pubmed11706043 Pubmed17556371 Pubmed18950639 Reactome Database ID Release 431675921 Reactome, http://www.reactome.org ReactomeREACT_121228 Reviewed: Wakelam, Michael, 2012-05-14