UBE2D1/2/3 UBE2D1,2,3:Ubiquitin Cytosolic Ubiquitination of HIF-alpha Authored: May, B, 2011-03-09 EC Number: 6.3.2.19 Edited: May, B, 2011-03-09 Pubmed10535940 Pubmed10878807 Pubmed10973499 Pubmed11454738 Pubmed12101228 Pubmed12538644 Pubmed9891082 Reactome Database ID Release 431234163 Reactome, http://www.reactome.org ReactomeREACT_120954 Reviewed: Rantanen, Krista, 2012-05-19 VHL is an E3 ubiquitin ligase that conjugates ubiquitin to hydroxylated HIF-alpha (Iwai et al. 1999, Kamura et al. 2000, Ohh et al. 2000, Groulx and Lee 2002, Maynard et al. 2003). VHL is predominantly cytosolic and shuttles between the cytosol and the nucleus (Lee et al. 1999, Groulx and Lee 2002). Ubiquitination and degradation of HIF-alpha can occur in both the cytosol and the nucleus (Berra et al. 2001). hydroxyPro-HIF-alpha:VHL:EloB/C:CUL2:RBX1 ub-hydroxyPro-HIF-alpha:VHL:EloB/C:CUL2:RBX1