Lanosterol is oxidatively demethylated to 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol Authored: Jassal, B, 2008-05-19 12:57:01 EC Number: 1.14.13.70 Edited: D'Eustachio, P, 2007-04-21 18:31:40 Lanosterol, NADPH + H+, and O2 react to form 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol, NADP+, H2O and formate. This oxidative demethylation reaction, in the endoplasmic reticulum, is catalyzed by lanosterol 14alpha-demethylase (CYP51A1). Although the reaction is annotated here as a single concerted event, studies with purified rat enzyme indicate that the methyl group is converted successively to an alcohol and an aldehyde before being released as formate (Trzaskos et al. 1986; Fischer et al. 1991; Gaylor 2002). Pubmed11969204 Pubmed2007571 Pubmed3771545 Pubmed8619637 Reactome Database ID Release 43194678 Reactome, http://www.reactome.org ReactomeREACT_10006 Reviewed: D'Eustachio, P, 2008-05-28 08:30:54 has a Stoichiometric coefficient of 3 has a Stoichiometric coefficient of 4 lanosterol + 3 NADPH + 3 H+ + 3 O2 => 4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol + 3 NADP+ + 4 H2O + formate