Cholesta-7,24-dien-3beta-ol is desaturated to form cholesta-5,7,24-trien-3beta-ol Authored: D'Eustachio, P, 2007-04-21 18:31:40 Cholesta-7,24-dien-3beta-ol, NADPH + H+, and O2 react to form cholesta-5,7,24-trien-3beta-ol, NADP+, and 2 H2O, catalyzed by SC5D. This reaction takes place in the endoplasmic reticulum. Its biochemical details are inferred from those of the reaction catalyzed by the purified rat protein (Kawata et al. 1985). The role of human SC5D in catalyzing this reaction in vivo is established from studies of patients in whom the enzyme is defective (Brunetti-Pierri et al. 2002; Krakowiak et al. 2003). Edited: D'Eustachio, P, 2007-04-21 18:31:40 Pubmed12189593 Pubmed12812989 Pubmed3997841 Reactome Database ID Release 43195664 Reactome, http://www.reactome.org ReactomeREACT_10092 Reviewed: Jassal, B, 2007-04-21 18:33:24 cholesta-7,24-dien-3beta-ol + NADPH + H+ + O2 => cholesta-5,7,24-trien-3beta-ol + NADP+ + 2 H2O has a Stoichiometric coefficient of 2