p-5Y-LAT:PLCG1 PLCgamma binds to p-5Y-LAT Authored: Garapati, P V, 2012-05-25 Edited: Garapati, P V, 2012-05-25 Pubmed10811803 Pubmed11390650 Pubmed20484116 Pubmed9830044 Reactome Database ID Release 432396606 Reactome, http://www.reactome.org ReactomeREACT_147817 Recruitment and activation of phospholipase C gamma (PLCgamma) is involved in DAP12 signal transduction. Phosphorylation of multiple substrates including PLCgamma1 has been observed in Ly49D/DAP12 triggered NK cells (McVicar et al. 1998). In myeloid cells, PLCgamma2 is recruited and then phosphorylated upon activation of TREM2 and DAP12 (Peng et al. 2010). PLCgamma is recruited to the plasma membrane by interactions with phosphorylated LAT mediated by the N-terminal SH2 domain of PLCgamma2, and by interactions with membrane phosphoinositides, mediated by the pleckstrin homology (PH) domain of PLCgamma2. Several studies have shown that LAT Y171 and Y191 also contribute to PLCgamma1 binding (Zhang et al. 2000). PLCgamma1 activity is dependent on the LAT-associated protein SLP-76 (Yablonski et al. 2001). Reviewed: Lanier, Lewis L, 2012-08-09