TetraHCA is conjugated with Coenzyme A (SLC27A2 VLCS) Authored: D'Eustachio, P, 2007-02-23 20:35:09 EC Number: 6.2.1.3 Edited: D'Eustachio, P, 2007-02-23 20:35:09 Pubmed10479480 Pubmed10749848 Pubmed11980911 Reactome Database ID Release 43193727 Reactome, http://www.reactome.org ReactomeREACT_10106 Reviewed: D'Eustachio, P, 2007-04-30 14:43:26 TetraHCA (25(R) 3alpha,7alpha,12alpha,24(S)-tetrahydroxy-5beta-cholestanoate), coenzyme A, and ATP react to form the CoA conjugate of 25(R) TetraHCA, AMP, pyrophosphate and water. This cytosolic reaction is catalyzed by SLC27A2 (VLCS). SLC27A5 (BACS) also catalyzes this reaction; the relative contributions of the two enzymes to de novo bile acid synthesis in vivo are not certain (Mihalik et al. 2002). TetraHCA + ATP + CoASH => 25(R) THCA-CoA + AMP + pyrophosphate + H2O (SLC27A2 "VLCS")