dsDNA:DAI:RIP1:RIP3 dsDNA:DAI DAI recruits RIP1 and RIP3 Authored: Shamovsky, V, 2011-09-21 Edited: Shamovsky, V, 2012-02-23 Pubmed18941233 Pubmed19590578 Reactome Database ID Release 431810457 Reactome, http://www.reactome.org ReactomeREACT_118851 Reviewed: D'Eustachio, P, 2011-12-07 Reviewed: Upton, JW, Mocarski, ES, 2012--0-2- Two RIP homotypic interaction motifs (RHIM) were identified in the DAI protein sequence. These two domains were shown to be essential for DAI-induced NFkB activation in human embryonic kidney 293T (HEK293T) cells. DAI forms a complex with two RHIM-containing kinases - RIP1 and RIP3 (Kaiser WJ et al 2008, Rebsamen M et al 2009). Recruitment of RIP3 to DAI was reported to induce RIP3 autophosphorylation. Furthermore, knockdown of RIP1 or RIP3 affected DAI-induced NFkB signals in murine L929 fibroblast and human HEK293T cells (Kaiser WJ et al 2008, Rebsamen M et al 2009).