K48-polyubiquitin TRAF3 dsRNA:RIG-1/MDA5:IPS-1:TRAF3 dsRNA:RIG-I/MDA5:IPS-1 K48-polyubiquitin Negative regulation of RIG-I/MDA5 signaling by TRIAD3A Authored: Garapati, P V, 2010-08-02 Edited: Garapati, P V, 2010-08-22 Pubmed19893624 Reactome Database ID Release 43936475 Reactome, http://www.reactome.org ReactomeREACT_24974 Reviewed: Kawai, T, Akira, S, 2010-10-30 TRAF3 is dual regulated by DUBA and TRIAD3A. TRAF3 K63-polyubiquitin is removed by DUBA to disrupt TRAF3-TBK1/IKKi interactions. TRAF3 then undergoes a late phase K48-linked polyubiquitination by TRIAD3A, leading to TRAF3 proteasomal degradation. Thus TRIAD3A acts as a E3- ubiquitin ligase that negatively regulates RLR pathway.