3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestanoate (DHCA) 3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al + NADPH + H+ + O2 => 3alpha, 7alpha-dihydroxy-5beta-cholestanoate (DHCA) + NADP+ + H2O 3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al, NADPH + H+, and O2 react to form 3alpha, 7alpha-dihydroxy-5beta-cholestanoate (DHCA), NADP+ and H2O. This oxidation reaction occurs in the mitochondrial matrix, catalyzed by CYP27A1. Authored: Jassal, B, 2007-01-19 10:34:59 Edited: D'Eustachio, P, 2007-02-17 19:40:28 Pubmed1708392 Pubmed9660774 Reactome Database ID Release 43193460 Reactome, http://www.reactome.org ReactomeREACT_10005 Reviewed: D'Eustachio, P, 2007-04-30 14:43:26